Ultra-mild bisulfite outperforms EM-seq for 5-methylcytosine detection with low input DNA
He, C.; Dai, Q.; Baldwin, T.; Lyu, R.; Daniels, B.; Ye, C.; Cao, C.; Zhu, C.; Fan, D.; Lin, L.; Liu, Y.; Wang, Y.
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We present Ultra-Mild Bisulfite Sequencing (UMBS-seq), a method for 5-methylcytosine (5mC) detection that minimizes DNA degradation and background noise. UMBS-seq outperforms conventional bisulfite and enzymatic methyl-sequencing (EM-seq) methods in library yield, complexity, and conversion efficiency when applied to low-input DNA samples. In particular, its effectiveness with low-input cell-free DNA (cfDNA) and hybridization-based target capture highlight its potential for clinical applications, including 5mC biomarker detection and early disease diagnosis.
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