Identification of human RNA Polymerase II interactors at early stages of transcription.
Ozer, A.; Kang, J. J.; Tang, Y.; Yugandhar, K.; Sun, Y.; DeBerardine, M. D.; Omar, K.; Lis, J.; Yu, H.
Show abstract
RNA Polymerase II (Pol II) transcription is highly regulated at two early steps in the transcription cycle: Pre-Initiation Complex (PIC) assembly with its coupled initiation, and promoter-proximal pausing with its controlled release. Here, we developed an optimized biochemical purification method that captures endogenously tagged chromatin-bound Pol II complexes under native conditions at these rate-limiting steps. We then identified a large set of Pol II interactors by mass spectrometry and determined the footprints of these assemblies on promoters with high resolution. Many well-known and new or understudied factors were identified as associated with the PIC and promoter-proximal paused complexes, indicating that despite decades of efforts, these rate-limiting steps of the transcription cycle are far from being completely understood. The new and understudied factors implicate novel mechanisms of regulation that will need to be characterized to fully understand Pol II regulation.
Matching journals
The top 3 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- RAPIDASH: A tag-free enrichment of ribosome-associated proteins reveals compositional dynamics in embryonic tissues and stimulated macrophages 97%
- Synaptic Activity Causes Minute-scale Changes in BAF Complex Composition and Function 97%
- Productive mRNA Chromatin Escape is Promoted by PRMT5 Methylation of SNRPB 97%
Similar papers in this journal
- DeepCLIP: Predicting the effect of mutations on protein-RNA binding with Deep Learning 96%
- The conserved elongation factor Spn1 is required for normal transcription, histone modifications, and splicing in Saccharomyces cerevisiae 96%
- Single-molecule imaging reveals a direct role of CTCF's zinc fingers in SA interaction and cluster-dependent RNA recruitment 96%
Similar papers in this journal
- TAF2 condensation in nuclear speckles links basal transcription factor TFIID to RNA splicing 98%
- Multi-omics and biochemical reconstitution reveal CDK7-dependent mechanisms controlling RNA polymerase II function at gene 5'- and 3'-ends 96%
- Asterix/Gtsf1 links tRNAs and piRNA silencing of retrotransposons 96%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.