Septin Architecture Dictates Size-Dependent Diffusion Barriers on biomimetic Membranes
Chauvin, B.; nakazawa, k.; Adriaans, i.; Geymonat, m.; piatti, s.; Hajj, B.; Mangenot, s.; Bertin, A.
Show abstract
Septins are cytoskeletal filaments bound to the inner leaflet of the plasma membrane, essential for cell division. They are involved in the formation of diffusion barriers for membrane-bound components. Whether septins directly function as barriers or as part of a broader regulatory cascade of events remains unclear. We addressed this using in vitro reconstituted assay of biomimetic synthetic membranes. We probed whether the diffusion of biomimetic fluorescent objects with tunable steric hindrance - mimicking membrane bound proteins - is constrained by septins. Our results indicate that (i) lipids and transmembrane proteins lacking cytosolic domains diffuse freely in the presence of septins (ii) membrane-bound objects with large cytosolic domains experience size-dependent diffusion constraints; and (iii) the ability of septins to act as diffusion barriers is finely tuned by their intrinsic filament organization. These findings demonstrate that septins can directly impose size-selective diffusion barriers and that their filament organization critically tunes this function.
Matching journals
The top 6 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Multivalent Interactions between Molecular Components Involved in Fast Endophilin Mediated Endocytosis Drive Protein Phase Separation 97%
- Lipid packing and cholesterol content regulate membrane wetting and remodeling by biomolecular condensates. 96%
- TMEM63 proteins act as mechanically-activated cholesterol modulated lipid scramblases contributing to membrane mechano-resilience 95%
Similar papers in this journal
- Ratiometric fluorescence nanoscopy and lifetime imaging of novel Nile Red analogs for analysis of membrane packing in living cells 95%
- Nanocluster-Mediated Signaling Crosstalk between FcγR and TLR4 in Macrophage Inflammatory Responses 94%
- Verifying molecular clusters by 2-color localization microscopy and significance testing 94%
Similar papers in this journal
Similar papers in this journal
- Lipid Packing Defects are Necessary and Sufficient for Membrane Binding of alpha-Synuclein 96%
- Combined thermodynamic and time-resolved structural analysis of interactions between AP2 and biomimetic plasma membranes provides insights into clathrin-mediated endocytosis 95%
- IM30 IDPs form a membrane protective carpet upon super-complex disassembly 94%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.