Ca2+ signature-dependent control of auxin sensitivity in Arabidopsis
Song, H.; Baudon, A.; Freund, M.; Randuch, M.; Pencik, A.; Ondrej, N.; He, Z.; Kaufmann, K.; Gilliham, M.; Friml, J.; Hedrich, R.; Huang, S.
Show abstract
Plants must continually balance growth with arrest, especially under stress. Auxin signaling acts as a central regulatory hub in this process, yet the mechanisms that dynamically tune auxin sensitivity in real time remain unknown. Here, we used the light-gated, Ca2+-permeable ChannelRhodopsin 2 variant XXM2.0 to optogenetically impose defined Ca2+ signatures on Arabidopsis root cells. Repetitive light activation triggered cytosolic Ca2+ signals that in turn suppressed auxin-induced membrane depolarization and Ca2+ transients. Moreover, persistent optogenetic Ca2+ stimulation affects auxin-responsive transcriptional reprogramming. As phenotypic output, reversible inhibition of cell division and elongation leading to root inhibition was observed. Our study thus introduces a new tool to decompose calcium-auxin crosstalk in plant cells, and demonstrates that optogenetically imposed cytosolic Ca2+ signals act as dynamic regulators of auxin susceptibility in roots.
Matching journals
The top 3 journals account for 50% of the predicted probability mass.
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.