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Monkeypox virus-neutralising antibodies detected against Clade Ib and Clade IIb in healthy individuals following MVA-BN vaccination

Sheridan, V. H.; Duffy, C. W.; Dunning, J.; Turtle, L.; Hiscox, J. A.; Subramaniam, K. S.; ISARIC4C Investigators,

2025-09-18 infectious diseases
10.1101/2025.09.17.25335983 medRxiv
Show abstract

Recently, a novel subclade of mpox virus (MPXV), clade Ib, has emerged in Eastern Democratic Republic of the Congo (DRC). Modified vaccinia Ankara--Bavarian Nordic (MVA-BN) is a third-generation smallpox vaccine that is authorised and in use as a vaccine against mpox. MVA-BN provides approximately 78% protection against disease to Clade IIb MPXV, but there are no data for Clade Ib. We used a plaque reduction neutralisation assay (PRNT) with MPXV clinical isolates from Clade Ib and IIb to investigate immunity in vaccinated health care workers. Here we show that a two-dose regimen of MVA-BN can induce MPXV-neutralising antibodies against both clades, with higher responses to Clade IIb than Clade Ib. KEY POINTSO_LIVaccination with MVA-BN generates antibodies that can neutralise MPXV Clade Ib and IIb. C_LIO_LIDespite the small sample size paired analysis across the vaccine recipients demonstrate higher neutralisation to Clade IIb than Ib. C_LI

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