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Simulating C3-to-CAM transition under ancient atmospheric conditions

Sarkar, D.; Kundu, S.

2025-09-17 plant biology
10.1101/2025.09.11.675551 bioRxiv
Show abstract

Crassulacean Acid Metabolism (CAM) plants can survive in arid environment. CAM is hypothesized to evolve from C3 metabolism about 20 to 30 million years ago (MYA) when the Earth faced declining atmospheric CO2 concentration ([CO2]a), increasing aridity and decreasing temperature. Understanding whether and how these atmospheric changes favour C3-to-CAM transition will help in engineering CAM into crop plants to tackle the threat of global climate change. Our simulations of plant cellular metabolism under the changing atmospheric conditions of ancient time observe C3-to-CAM transition, capture possible temporal alterations of active metabolic pathways and further confirm that both the reduced [CO2]a and increased water scarcity associated with higher aridity, can act as evolutionary agents, driving the C3-to-CAM transition. Although the predicted elevated [CO2]a of future reveals a reversion towards C3-like behaviour, drought always favours CAM regardless of [CO2]a and temperature levels. Moreover, a minimum oxygen concentration is needed to support higher nocturnal respiration, necessary for CAM.

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