NeuronID: An automatic toolkit for identifying neurons in two-photon calcium imaging data
Peng, J.; Xu, T.
Show abstract
Two-photon calcium imaging has emerged as a powerful technique for monitoring neuronal activity in neuroscience; however, its data processing remains challenging. Here, we introduce NeuronID, an automatic toolkit designed to process two-photon calcium imaging data. The NeuronID toolkit features a modular architecture that includes motion correction, noise reduction, segmentation of neuronal components, and extraction of neuronal signals. Notably, the NeuronID toolkit offers an optimized strategy for segmenting neuronal components, which systematically integrates morphological boundary identification, cross-correlation analysis between pixels, and evaluation of neuronal signal quality. Compared to existing tools or manual annotation by experts, the NeuronID toolkit reduces the likelihood of over-segmentation while achieving near-human accuracy. Overall, this study provides a standardized analytical tool for processing two-photon calcium imaging data.
Matching journals
The top 2 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Minutes-timescale 3D isotropic imaging of entire organs at subcellular resolution by content-aware compressed-sensing light-sheet microscopy 96%
- Light microscopy based approach for mapping connectivity with molecular specificity 96%
- Dense, Continuous Membrane Labeling and Expansion Microscopy Visualization of Ultrastructure in Tissues 96%
Similar papers in this journal
- A spectral demixing method for high-precision multi-color localization microscopy 96%
- 3D-Aligner: An advanced computational tool designed to correct image distortion in expansion microscopy for precise 3D reconstitution and quantitative analysis 95%
- Fast volumetric fluorescence lifetime imaging of multicellular systems using single-objective light-sheet microscopy 95%
Similar papers in this journal
- Extended field-of-view ultrathin microendoscopes for high-resolution two-photon imaging with minimal invasiveness in awake mice 96%
- DeCalciOn: A hardware system for real-time decoding of in-vivo calcium imaging data 96%
- Longitudinal tracking of neuronal activity from the same cells in the developing brain using Track2p 96%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.