Etiological spectrum of non-compressive myelopathies in Uganda: a prospective, observational study using neuroimaging, autoantibodies, and next-generation sequencing

BackgroundNon-compressive myelopathy can lead to severe disability and death. The diagnosis is often complex and resource-intensive, with regional variation in causes. Data on the epidemiology and etiology of non-compressive myelopathy in Africa remains limited. MethodsA prospective observational study of adults presenting with clinical signs and symptoms of non-compressive myelopathy was conducted. Patients were recruited from 2013-2015 and 2018-2022 in Kampala, Uganda. Participants underwent spinal magnetic resonance imaging (MRI) to exclude extradural spinal cord lesions. Serum and cerebrospinal fluid (CSF) were tested for aquaporin-4 (AQP4) and myelin oligodendrocyte glycoprotein (MOG) antibodies. Metagenomic next-generation sequencing (mNGS) was performed on CSF to detect infectious etiologies. Serum vitamin B12 levels and tissue biopsies were performed at clinician discretion. Participant characteristics and diagnostic findings were summarized using descriptive statistics. FindingsAmong 420 participants screened, 144 were enrolled and included in this analysis. The median age was 33 years (interquartile range 25-44). 79 participants (55%) were male, and 38 (26%) were living with HIV. Intradural abnormalities were identified on spinal MRI in 79 (55%) participants. An etiologic diagnosis was established in 50 (35%) participants, including neuromyelitis optica spectrum disorder (NMOSD, n=10), myelin oligodendrocyte glycoprotein antibody-associated disease (MOGAD, n=7), Schistosoma mansoni (n=9), cytomegalovirus (n=1), varicella zoster virus (n=1), vitamin B12 myelopathy (n=8), spinal cord tumor (n=12), and arteriovenous malformation (n=2). InterpretationThis study identified a wide range of etiologies for non-compressive myelopathy in Uganda. Autoimmune myelopathies (NMOSD and MOGAD) accounted for 14% of cases, while mNGS identified an infectious cause in 13% of participants. These findings highlight the importance of expanding access to both autoimmune and infectious diagnostic testing in resource-limited settings. FundingThis study was supported by the NIH (R01NS113828, R01AI145437, R25TW009345, K24AI096925, and 1K43TW010718) and the Westridge Foundation (MRW). Sequencing was performed at the UCSF Center for Advanced Technology, supported by UCSF PBBR, RRP IMIA, and NIH 1S10OD028511-01 grants. The K24AI096925 was used in the design of the study and data collection, R25TW009345 was used in data collection while 1K43TW010718 was used in analysis, interpretation of the data and in writing the manuscript.