Medium-chain fatty acids mitigate endoplasmic reticulum stress in yeast cells
Ishiwata-Kimata, Y.; Kimata, Y.
Show abstract
Upon endoplasmic reticulum (ER) stress, eukaryotic cells commonly trigger cytoprotective transcriptome changes, namely the unfolded protein response (UPR). In yeast Saccharomyces cerevisiae, the UPR is mediated by the transcription factor Hac1, which is induced in response to ER stress. Since Hac1 controls hundreds of genes, the biological phenomena that result from UPR are not yet fully understood. Here, we show that cells carrying a mutation to constitutively express Hac1 abundantly contained C10:0 and C12:0 fatty acids, known as medium-chain fatty acids (MCFs). UPR induction by some ER stress stimuli was attenuated by externally supplied MCFs in cells in which fatty acid elongation was genetically or pharmacologically halted. The cell survival assay also indicated the mitigation of ER stress by the MCFs. Moreover, we demonstrated that MCFs leads to the diffusion of a mutant transmembrane protein aggregated in the ER. We propose that, as a biologically beneficial outcome of UPR, MCFs are produced to change the properties of the ER membrane, such as fluidity, in ER-stressed yeast cells.
Matching journals
The top 12 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Pex24 and Pex32 tether peroxisomes to the ER for organelle biogenesis, positioning and segregation 95%
- Multiple ammonium transporters in fission yeast are coordinated by transcriptional and localization regulation in response to nitrogen starvation 94%
- An improved tetracycline-inducible expression system for fission yeast 94%
Similar papers in this journal
- Physicochemical properties of the vacuolar membrane and cellular factors determine formation of vacuolar invaginations 94%
- A Rab Escort Protein Regulates the MAPK Pathway That Controls Filamentous Growth in Yeast 94%
- Removing auto-activators from yeast-two-hybrid assays by conditional negative selection 94%
Similar papers in this journal
- Heat Stress Regulates the Expression of TPK1 Gene at Transcriptional and Post-Transcriptional Levels in Saccharomyces cerevisiae 95%
- New insights into the functions of ACBD4/5-like proteins using a combined phylogenetic and experimental approach across model organisms 94%
- Intracellular level of S. cerevisiae Rad51 is regulated via proteolysis in a SUMO- and Ubiquitin-dependent manner 94%
Similar papers in this journal
- Deletion of sulfate transporter SUL1 extends yeast replicative lifespan via reduced PKA signaling instead of decreased sulfate uptake 94%
- High and stable ATP levels prevent aberrant intracellular protein aggregation 93%
- OXPHOS deficiencies affect peroxisome proliferation by downregulating genes controlled by the SNF1 signaling pathway 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.