Tech Note - Adaptive Sampling: targeted Oxford Nanopore long-read sequencing
Crang, N.; Contreras Lopez, O.; Olsen, R.-A.; Hasby, F.
Show abstract
Oxford Nanopore Technology (ONT) has developed a long-read sequencing method known as "adaptive" or "Read Until" sequencing. Unlike Illumina sequencing, this ONT read data is available in real-time during the run, rather than only being available when the sequencing run is complete and it focuses on a specified region of interest (ROI) within the sequence data. We investigated how this technique compared to standard sequencing under a variety of conditions and found it consistently gave a multifold increase in coverage of the ROI compared to standard sequencing, though a strong positional effect was observed for ROI efficacy. Adaptive sampling can be used to selectively enrich or deplete sequences from a library in real-time, enhancing coverage of regions of interest without requiring additional library pre-processing
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