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Immune cell de novo steroidogenesis regulates inflammation resolution and recovery in acute lung injury

Hussein, H. A. M.; Shaji, S. K.; Veiga-Villauriz, C.; Chakraborty, S.; Pramanik, J.; Ali, F. A. Z.; Yuan, J.; Khanfar, E.; Makuyana, N.; Ali, Y. M.; Zhao, Q.; Hebenstreit, D.; Mahata, B.

2025-09-04 immunology
10.1101/2025.08.29.673039 bioRxiv
Show abstract

Effective resolution of inflammation following acute lung infection or injury is critical for restoring immune and tissue homeostasis to ensure functional recovery. Prolonged or unresolved inflammation can impair lung repair, promote fibrosis, and contribute to pulmonary dysfunction. While systemic steroid signalling is known to modulate general immune responses, the specific role of immune cell-mediated steroidogenesis in regulating lung inflammation and repair remains unknown. Here, we show that immune cell de novo steroidogenesis is essential for resolving inflammation and promoting recovery in a murine model of acute lung injury. During the resolution phase, steroid-synthesizing immune cells, predominantly basophils, are enriched in the lung. Mice with immune cell-specific ablation of de novo steroidogenesis exhibit exacerbated lung injury, impaired resolution of inflammation, and defective tissue repair. These findings reveal a previously unrecognized immunoregulatory function of immune cell-derived steroids and identify immune cell steroidogenesis as a potential therapeutic target for promoting resolution and recovery in inflammatory lung diseases.

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