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Genetically-based sperm discrimination in the vaginal tract of a primate species

Petersen, R. M.; Nonnamaker, L. M.; Anderson, J. A.; Bergey, C. M.; Roos, C.; Melin, A. D.; Higham, J. P.

2025-09-04 evolutionary biology
10.1101/2025.08.29.672970 bioRxiv
Show abstract

Females influence offspring paternity through diverse pre- and post-copulatory mechanisms. Sperm discrimination--the differential physiological response to ejaculates based on male or sperm characteristics--can bias fertilization outcomes, but in vivo evidence of this process in large-bodied mammals is lacking. Here, we tested whether two aspects of female physiology that affect sperm survival-vaginal immune response and pH-are modulated by male genetic makeup in a nonhuman primate. We observed post-copulatory differences in vaginal gene expression and pH, with the strongest immune responses and largest pH decreases, harmful to sperm, exhibited by females mating with genetically similar males. These findings support genetically based post-copulatory mate discrimination in primates, shedding light on the interaction between male gametes and the female reproductive tract and potentially explaining variation in conception probability.

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