Scaffolded hiPSC liver organoids recapitulating bile duct tubulogenesis and periportal architecture.
Rajendiran, H.; Luciani, F.; Serhrouchni, S.; Monet, A.; Ong, H. T.; Zhu, J.; Mishra, P.; Marchand, M.; Sahni, G.; Arora, A.; Nai, B. M. H.; Hepburn, M. S.; LI, J.; Mowla, A.; Navaeipour, F.; Kennedy, B.; Viasnoff, V.
Show abstract
Recapitulating the liver periportal area in vitro remains a major challenge due to its complex cellular composition and the coordinated development of both ductal and endothelial networks. Most existing bile duct organoid models fail to reproduce tubular extension and multicellular organization. We present a 3D ECM micro-rods system biofunctionalized with grafted growth factors mimicking fetal liver paracrine signaling to initiate and to coordinate the development of bile ducts, parenchymal cells, and vascular structures. Our system recapitulates key developmental stages, from the formation of the ductal plate to the emergence of elongated tubular ducts. The ECM rod alone provides the biophysical cues initiating the elongation of the bile ducts and the spatial structuration of the cellular organization, while the biofunctionalization with growth factors enhance the organization and maturation of the biliary and vascular systems. We further demonstrate that this approach is scalable and amenable to quantitative analysis through label-free Optical Coherence Tomography combined with AI-driven 3D segmentation.
Matching journals
The top 8 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Recapitulating apicobasal tissue polarity in extracellular matrix incorporated airway organoids 94%
- Human macrophage polarization determines bacterial persistence of Staphylococcus aureus in a liver-on-chip-based infection model 93%
- Liver ductal organoids reconstruct intrahepatic biliary trees in decellularized liver grafts 93%
Similar papers in this journal
- Development of a hepatic cryoinjury model to study liver regeneration 93%
- LiverZap: A chemoptogenetic tool for global and locally restricted hepatocyte ablation to study cellular behaviours in liver regeneration 93%
- Next-Generation Breast Organoids Capture Tissue Organogenesis with High-Resolution Live Imaging 91%
Similar papers in this journal
Similar papers in this journal
- Temporal Analyses of Postnatal Liver Development and Maturation by Single Cell Transcriptomics 93%
- Differentiation of human intestinal organoids with endogenous vascular endothelial cells 93%
- A combined transcriptional and dynamic roadmap of single human pancreatic endocrine progenitors reveals proliferative capacity and differentiation continuum 91%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.