Restricted edition of ALS is required during co-edition in order to maintain normal tomato development
Kuroiwa, K.; Laurens, A.; Corre, M.-N.; Lugan, R.; Mazier, M.; Gallois, J.-L.
Show abstract
Co-editing strategies have emerged as an approach to facilitate the selection of CRISPR/Cas-mediated mutants in a transgene-free manner: the gene of interest is edited together with a reporter gene, whose mutation can be selected visually or pharmacologically. In this work, we asses the impact of editing the well-used reporter Acetolactate synthase (ALS) on plant development and metabolome. We show that the desired mutation in ALS1 (P186S) conferring selectable herbicide resistance trait does not show significant impact on the plant morphology and physiology but that the additional mutations resulting from the same sgRNA can result in reduced vegetative vigor and altered metabolomic profiles in tomato.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Dually biofortified cisgenic tomatoes with increased flavonoids and branched-chain amino acids content 96%
- Modulating activity of the APC/C regulator SlSAMBA improves the sugar and antioxidant content of tomato fruits 96%
- Highly efficient homology-directed repair using transient CRISPR/Cpf1-geminiviral replicon in tomato 95%
Similar papers in this journal
- Seeing the unseen: A trifoliate (MYB117) mutant allele fortifies folate and carotenoids in tomato fruits 95%
- WHIRLY1 regulates aliphatic glucosinolate biosynthesis in early seedling development of Arabidopsis 95%
- MdLRR-RLK1-MdATG3 module enhances the resistance of apples to abiotic stress via autophagy 95%
Similar papers in this journal
Similar papers in this journal
- Whole-Genome Profiling of Ethyl Methanesulfonate Mutagenesis in Tomato 96%
- Generation and molecular characterization of CRISPR/Cas9-induced mutations in 63 immunity-associated genes in tomato reveals specificity and a range of gene modifications 95%
- Highly efficient generation of canker resistant sweet orange enabled by an improved CRISPR/Cas9 system 94%
Similar papers in this journal
- Introgression of a dominant phototropin1 mutant superenhances carotenoids and boosts flavor-related volatiles in genome-edited tomato RIN mutants 95%
- Several geranylgeranyl diphosphate synthase isoforms supply metabolic substrates for carotenoid biosynthesis in tomato 95%
- Asparagine accumulation in chicory storage roots is controlled by translocation and feedback regulation of asparagine biosynthesis in leaves 94%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.