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spammR: an R package designed for analysis and integration of spatial multi-omic measurements

Mahlich, Y.; Sohi, H.; Piehowski, P.; McDermott, J. E.; Gosline, S. J.

2025-08-28 bioinformatics
10.1101/2025.08.26.672472 bioRxiv
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SummarySpatial omics is a young and evolving field and as such shows rapid development of novel technologies and analysis methods to measure transcripts, proteins, metabolites, and post-translational modifications at high spatial resolution. These advances in technology have enabled the simultaneous generation of abundance profiles for multiple different omics types and associated microscopy imaging data, as well as their analysis in a spatial context. However, most analytical tools are designed for spatial transcriptomics platforms and are challenging to use in other contexts such as mass spectrometry-based measurements or metagenomics. To this end we present spammR (spatial analysis of multi-omics measurements in R), an R package that enables end-to-end analysis with a specific focus on mass-spectrometry derived spatial omics datasets with the goal of integration across multiple data types (e.g. sequencing, metabolites, proteins) within the same tissue sample. Availability and implementationspammR is implemented in R. The package is currently installable from GitHub (https://github.com/PNNL-CompBio/spammR).

Published in Bioinformatics Advances (predicted rank #2) · training set

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