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Efficient Double Helix Detection with Steerable Filters

Barentine, A. E. S.; Balaji, A.; Moerner, W. E.

2025-08-20 biophysics
10.1101/2025.08.14.670427 bioRxiv
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We present an efficient detection scheme for localization of Double Helix point-spread functions for 3D single-molecule localization microscopy or tracking. Using steerable filters, we extract both 2D position and lobe orientation (axial position) estimates using just 7 convolutions, orders of magnitude less than used in deep learning based approaches. For a complete SMLM analysis pipeline, we pair this detection with a fitter using an optimally parameterized double Gaussian model, and implement both as a plugin for the open source PYthon Microscopy Environment (PYME).

Published in Biomedical Optics Express (predicted rank #4) · training set

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