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Taurine Inhibits Apolipoprotein E4 Aggregation

Legrand, A.; Cerna, K.; Marques, S. M.; Verma, N.; Kopko, J.; Vanova, T.; Subramanian, M.; Jaroslav, B.; Henek, T.; Vanacek, P.; Kucera, J.; Iglesias, J. P.; Sedmik, J.; Pospisilova, V.; Kouba, P.; Vaskova, A.; Nezvedova, M.; Sedlar, J.; Damborsky, J.; Mazurenko, S.; Marek, M.; Sivic, J.; Hernychova, L.; Bednar, D.; Bohaciakova, D.; Prokop, Z.

2025-08-13 biochemistry
10.1101/2025.08.13.669519 bioRxiv
Show abstract

Apolipoprotein E4 (ApoE4) is a major genetic risk factor in many neurodegenerative diseases, yet effective therapeutic strategies targeting its associated pathologies remain unresolved. The aggregation of ApoE4, a key pathological feature, can be attenuated by tramiprosate and its metabolite 3-sulfopropanoic acid. In this study, we investigated the potential of taurine, a close chemical analogue of tramiprosate, to modulate ApoE4-mediated pathological processes. Using an integrated approach--including molecular dynamics simulations, static light scattering, mass spectrometry, and cerebral organoid models--we investigated taurines effects on ApoE4 aggregation. We found that taurine effectively inhibits ApoE4 aggregation. Notably, taurine significantly ameliorates the pathophysiological characteristics of ApoE4, bringing its phenotype closer to the more benign ApoE3 variant. By leveraging its neuroprotective properties, taurine may offer effects comparable to tramiprosate and 3-sulfopropanoic acid, positioning it as an accessible and promising candidate for mitigating neurodegeneration, particularly in individuals with the high-risk ApoE4/E4 genotype.

Published in Biomedicine & Pharmacotherapy · training set

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