Time to embrace the molecular approach for respiratory pathogen screening
Rodrigues, A.; Cazelli, M. E.; Eulalio, M.; Ferreira, S.; Sousa, A.
Show abstract
The current gold standard for clinical pathogen identification relies on a combination of culture-based methods, target-specific molecular techniques, and antigen-detection assays1. Known limitations of the first method include the requirement for viable and culturable microorganisms, while the second approach is constrained to a limited number of predefined targets. These constraints reduce their diagnostic success, as not all pathogens are culturable, easily isolated from commensal microbiota, or represented in the selected detection panels. While culture-based methods are widely recognized for their limited sensitivity, updated quantifications of their actual performance in real-world clinical settings are scarce. To address this gap, we conducted a retrospective survey of all lower respiratory tract (LRT) samples processed by either culture or target-based molecular methods over a five-year period at a Portuguese hospital centre, providing a quantitative assessment of current diagnostic performance.
Matching journals
The top 9 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Evaluating the feasibility, sensitivity, and specificity of next-generation molecular methods for pleural infection diagnosis 95%
- Avoiding false positive SARS-CoV-2 rapid antigen test results with point-of-care molecular testing on residual test buffer 93%
- SARS-CoV-2 antibodies rapid tests: a valuable epidemiological tool in challenging settings 93%
Similar papers in this journal
- Metagenomic sequencing to detect respiratory viruses in persons under investigation for COVID-19 94%
- Diagnostic accuracy study of a novel blood-based assay for identification of TB in people living with HIV 93%
- Metagenomic sequencing as a clinical diagnostic tool for infectious diseases: a systematic review and meta-analysis 93%
Similar papers in this journal
- Development of a qualitative real-time RT-PCR assay for the detection of SARS-CoV-2: A guide and case study in setting up an emergency-use, laboratory-developed molecular assay 90%
- Molecular point-of-care testing for influenza A/B and respiratory syncytial virus: comparison of workflow parameters for the ID Now and cobas Liat systems 89%
- Increasing both specificity and sensitivity of SARS-CoV-2 antibody tests by using an adaptive orthogonal testing approach 89%
Similar papers in this journal
- Combined oropharyngeal/nasal swab is equivalent to nasopharyngeal sampling for SARS-CoV-2 diagnostic PCR 91%
- An optimised protocol for detection of SARS-CoV-2 in stool 89%
- Full-length 16S rRNA gene amplicon analysis of human gut microbiota using MinION™ nanopore sequencing confers species-level resolution 89%
Similar papers in this journal
- A needle in a haystack: metagenomic DNA sequencing to quantify Mycobacterium tuberculosis DNA and diagnose tuberculosis 94%
- Pooling RT-PCR test of SARS-CoV-2 for large cohort of 'healthy' and infection-suspected patients: A prospective and consecutive study on 1,000 individuals 91%
- PathCrisp : An Innovative Molecular Diagnostic Tool for Early Detection of NDM-Resistant Infections 91%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.