Foliar mRNA spray induces protein synthesis in monocot crop and dicot model plant species
Uslu, V. V.; Dobrowitsch, M.; Preussel-Danger, K.; Furch, A. C. U.; Noetzold, J.; Richter, A. M.; Schlemmer, T.; Dong, Y.; Krczal, G.; Koch, A.
Show abstract
In planta gene expression via exogenous mRNAs has a wide range of potential biotechnological applications from genome editing to plant protection and to circumventing tissue culture. Yet, regarding mRNA delivery into intact plant cells, the purity, stability and translational efficiency of exogenous mRNAs appear as obstacles for effective exogenous mRNA application. Our study investigates the potential of exogenous mRNAs in mediating protein synthesis in plant cells. We applied unformulated, unmodified green fluorescent protein (GFP)-coding mRNA to monocot (Triticum aestivum, Hordeum vulgare, Zea mays) crops and dicot model plant Arabidopsis thaliana and observed GFP signals in all species, confirming the successful uptake and translation of mRNA into protein. This approach opens possibilities for broader, GM-free applications in plant science.
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