Multivesicular release at a small central excitatory synaptic contact
Barbour, B.; Lohof, A. M.
Show abstract
We set out to study the properties of excitatory synaptic transmission at a central connection likely to consist of a single small synaptic contact. In the cerebellar cortex, molecular layer interneurones extend sparse thin dendrites in a parasagittal plane. The nonbranching parallel fibres traverse at right angles to the dendritic plane, forming en passant excitatory synapses. This arrangement makes it very likely that any synaptic connection between a parallel fibre and an interneurone will involve only a single contact. We obtained 20 paired recordings of synaptically connected granule cells and interneurones in cerebellar slices of immature rat, recorded at room temperature. Three of these pairs were successfully labelled using biocytin and a single point of contact between them was indentified. In 19/20 recordings, multiple release events corresponding to asynchronously released vesicles were frequently resolved following a single action potential. The frequency of these events could not be accounted for by spontaneous EPSCs, whose frequency was very low. We conclude that at this excitatory synaptic contact an action potential can release more than one vesicle.
Matching journals
The top 6 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Glutamatergic Control Of A Pattern-Generating Central Nucleus In A Gymnotiform Fish 96%
- Resolution of subcomponents of synaptic release from post-synaptic currents in rat hair-cell/auditory-nerve fiber dendrites 95%
- The dynamic range of voltage-dependent gap junction signaling is maintained by Ih-induced membrane potential depolarization 94%
Similar papers in this journal
- Stellate cell computational modelling predicts signal filtering in the molecular layer circuit of cerebellum 94%
- Kv1 potassium channels control action potential firing of putative GABAergic deep cerebellar nuclear neurons. 94%
- Local field potential sharp waves with diversified impact on cortical neuronal encoding of haptic input 94%
Similar papers in this journal
- Transport-Related Effects on Intrinsic and Synaptic Properties of Human Cortical Neurons: A Comparative Study 94%
- Intrinsic mechanisms contributing to the biophysical signature of mouse gamma motoneurons 94%
- Ion-channel degeneracy and heterogeneities in the emergence of complex spike bursts in CA3 pyramidal neurons 94%
Similar papers in this journal
- Postnatal Development Of Dendritic Morphology And Action Potential Shape In Rat Substantia Nigra Dopaminergic Neurons 95%
- Sub-optimal Discontinuous Current-Clamp switching rates lead to deceptive mouse neuronal firing 95%
- Group II metabotropic glutamate receptors modulate sound evoked and spontaneous activity in the mouse inferior colliculus 94%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.