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AgRP Neuronal Suppression by Spontaneous Movement Encodes Exercise Reinforcement

Yoshida, J.; Suematsu, N.; Soma, S.; Levy, I.; Horvath, T.

2025-08-09 neuroscience
10.1101/2025.08.06.668910 bioRxiv
Show abstract

Hypothalamic agouti-related peptide (AgRP) neurons that drive hunger-related behaviors are activated during energy deficit, encode negative valence but can be inhibited even by perception of food without digestion. Here, we show that in head-fixed mice, discrete motor actions such as spontaneous walking and conditioned licking rapidly suppress AgRP neuron activity. This suppression persisted throughout movement and terminated upon cessation, which was independent of food perception or conditioned cues. The degree of suppression was positively correlated with movement vigor. While optogenetic enhancement of AgRP neuron suppression did not initiate movement, it promoted reinforcement of concurrent walking behavior. These findings unmask that spontaneous, low intensity physical activity can reduce AgRP neuron activity reinforcing concurrent movement/exercise with implications for systemic health benefits.

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