Mycobacterial DNA-binding protein 1 (MDP1) induces RNA condensation as revealed by high-speed AFM
Shoukat, N.; Nishiyama, A.; Nakamoto, K.; Goto, Y.; Nguyen, P. D. N.; Ichida, H.; Umeda, K.; Matsumoto, S.; Kodera, N.
Show abstract
Mycobacterial DNA-binding protein 1 (MDP1) is a histone-like protein in Mycobacterium tuberculosis (Mtb) that contributes to genome organization and dormancy adaptation. MDP1 consists of a structured HU-like region (HUR), and a post-translational modifications (PTMs)-enriched intrinsically disordered region (IDR), that regulate its function. While its role in DNA condensation is well established, how MDP1 interacts with RNA remains unclear, despite growing recognition of transcriptional regulation during dormancy. Here, by using total RNA from E. coli as a model substrate, we investigated whether MDP1 induces RNA condensation. Our high-speed AFM and optical microscopy analysis shows that native MDP1 from Mtb, rich in PTMs, forms globular RNA condensates. In contrast, MDP1 expressed in E. coli, which lacks PTMs, induces chain-like RNA condensates. Domain-specific analysis revealed that the IDR, the synergy between the IDR and HUR, and PTMs are essential for MDP1-induced RNA condensation. These findings suggest that MDP1 mediates RNA organization during dormancy.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Chromatin sequesters pioneer transcription factor Sox2 from exerting force on DNA 95%
- Phosphorylation Toggles the SARS-CoV-2 Nucleocapsid Protein Between Two Membrane-Associated Condensate States 95%
- Smart 3D super-resolution microscopy reveals the architecture of the RNA scaffold in a nuclear body 95%
Similar papers in this journal
- The human RAD52 complex undergoes phase separation and facilitates bundling and end-to-end tethering of RAD51 presynaptic filaments 95%
- Roles of dimeric intermediates in RNA-catalyzed rolling circle synthesis 95%
- Dynamic ParB-DNA interactions initiate and maintain a partition condensate for bacterial chromosome segregation 95%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.