Terminal Loop Sequences in Viral Double-Stranded RNAs Modulate RIG-I Signaling
Hackbart, M.; Wang, P.; Gnazzo, V.; Lopez, C. B.
Show abstract
Detection of foreign RNAs is a crucial activation step for innate immunity pathways in response to viral infections. Retinoic acid-inducible gene I (RIG-I) is a cytoplasmic RNA sensor that triggers type I and III interferon (IFN) expression and activates the antiviral response in response to RNA virus infection. The activating ligand for RIG-I has been shown to be 5-triphosphated, blunt-ended, double-stranded (ds)RNA, but questions remain on the impact of other RNA motifs on RIG-I activation. Here we show that immune-activating copy-back viral genomes (cbVGs) contain RNA stem loops away from the 5 end of the RNA that enhance RIG-I signaling and IFN expression. Importantly, the sequence of the terminal loops of the activating motifs impacts the strength of IFN expression. Additionally, we show that synthetic versions of these cbVG-derived stem loops trigger innate immune responses in mice demonstrating their potential as immunostimulants in vivo.
Matching journals
The top 5 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Small Molecule Targeting IRES Domain Inhibits Enterovirus 71 Replication via an Allosteric Mechanism that Stabilizes a Ternary Complex 94%
- A conserved long-range RNA interaction in SARS-CoV-2 recruits ADAR1 to enhance virus proliferation 94%
- Tick-borne flavivirus exoribonuclease-resistant RNAs contain a 'double loop' structure 93%
Similar papers in this journal
- Designed Nanoparticles Elicit Cross-Reactive Antibody Responses To Conserved Influenza Virus Hemagglutinin Stem Epitopes 94%
- Identifying cellular RNA-binding proteins during infection uncovers a role for MKRN2 in influenza mRNA trafficking 93%
- Characterisation of the Semliki Forest Virus-host cell interactome reveals the viral capsid protein as an inhibitor of nonsense-mediated mRNA decay 93%
Similar papers in this journal
- Comparison of immunogenicity and protection efficacy of self-amplifying and circular mRNA vaccines for SARS-CoV-2 94%
- Characterization of SARS-CoV-2 N protein reveals multiple functional consequences of the C-terminal domain 94%
- Integrated role of microRNA-30e-5p through targeting negative regulators of innate immune pathways during HBV infection and SLE 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.