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MR-AIV reveals in-vivo brain-wide fluid flow with physics-informed AI

Toscano, J. D.; Guo, Y.; Wang, Z.; Mori, Y.; Vaezi, M.; Karniadakis, G. E.; Boster, K. A. S.; Kelley, D. H.

2025-08-01 neuroscience
10.1101/2025.07.30.667741 bioRxiv
Show abstract

The circulation of cerebrospinal and interstitial fluid plays a vital role in clearing metabolic waste from the brain, and its disruption has been linked to neurological disorders. However, directly measuring brain-wide fluid transport--especially in the deep brain--has remained elusive. Here, we introduce magnetic resonance artificial intelligence velocimetry (MR-AIV), a framework featuring a specialized physics-informed architecture and optimization method that reconstructs three-dimensional fluid velocity fields from dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI). MR-AIV unveils brain-wide velocity maps while providing estimates of tissue permeability and pressure fields--quantities inaccessible to other methods. Applied to the brain, MR-AIV reveals a functional landscape of interstitial and perivascular flow, quantitatively distinguishing slow diffusion-driven transport ([~] 0.1 {micro}m/s) from rapid advective flow ([~] 3 {micro}m/s). This approach enables new investigations into brain clearance mechanisms and fluid dynamics in health and disease, with broad potential applications to other porous media systems, from geophysics to tissue mechanics.

Published in Science Advances (predicted rank #16) · training set

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