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Analyzing the T cell receptor repertoire of 2,804 individuals with inflammatory bowel disease identifies public T cell responses involved in the pathogenesis

ElAbd, H.; Mahdy, A. K. H.; Kokubun, E. E.; Kriukova, V.; Olbjorn, C.; Perminow, G.; Bengtson, M.-B.; Ricanek, P.; Andersen, S.; Detlie, T. E.; Kristensen, V.; Hov, J. R.; Hoivik, M. L.; Franke, A.

2025-07-29 immunology
10.1101/2025.07.24.666514 bioRxiv
Show abstract

Whereas altered immune processes have been identified in individuals with inflammatory bowel disease (IBD), potentially causative antigens remain to be identified. By interrogating the immune repertoire of individuals with IBD, an identification of common antigenic exposures associated with the disease can be obtained. We analyzed the T cell receptor beta (TRB) chain repertoire of 1,890 individuals with Crohns disease (CD) and 914 individuals with ulcerative colitis (UC), enabling the identification of 327 TRB clonotypes associated with CD and 130 with UC. We validated the expansion of these clonotypes in a cohort of treatment-naive individuals with either CD, UC or symptomatic control (n=855). These disease-associated clonotypes were restricted to disease-associated risk HLA alleles and their expansion correlated with disease-severity but not with surgery or treatment trajectory. In conclusion, we identified and validated TRB clonotypes that are associated with either CD or UC, these clonotypes are a novel therapeutic target in IBD.

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