Reliability and signal comparison of OPM-MEG, fMRI & iEEG in a repeated movie viewing paradigm

Optically pumped magnetometers (OPMs) offer a promising advancement in noninvasive neuroimaging via magnetoencephalography (MEG), but establishing their reliability and comparability to existing methods remains an ongoing endeavor. Here, we evaluated OPM recordings by assessing their test-retest reliability and comparing them to functional magnetic resonance imaging (fMRI) and intracranial electroencephalography (iEEG) recordings. Data were collected from three independent participant groups during repeated viewings of a movie segment. In 7 canonical frequency bands ({delta}: 0.5-4 Hz,{theta} : 4-8 Hz, : 8-12 Hz, {beta}: 12-28 Hz,{gamma} 1: 28-46 Hz,{gamma} 2: 55-70 Hz, and HF: 64-116 Hz), as well as a broadband (BB: 0.5-116 Hz) signal, we quantified the signal consistency (1) within individuals, (2) across subjects, and (3) across modalities. OPM exhibited widespread reliability, particularly in lower frequency bands; spatial patterns resembled those of fMRI and iEEG in visual and auditory regions. Cross-modal analyses revealed robust correspondence between OPM and both fMRI and iEEG, including inverse correlations at low frequencies and positive correlations at higher frequencies in the OPM-fMRI comparison, consistent with known relationships between oscillatory power and BOLD responses. Comparisons of signal-to-noise (SNR) estimates further revealed that in some regions, the SNR of cross-modal alignment exceeded within-modality reliability, suggesting that bridging between modalities can sometimes enhance SNR by attenuating reliably shared noise. Our findings demonstrate that OPM consistently captures stimulus-locked neural dynamics that converge with established modalities.