TRIM28 regulates pre-mRNA splicing via phosphorylation and SUMOylation networks
Matkovic, V.; Prieto-Garcia, C.; Mosler, T.; Wagner, K.; Hotz, P.; Haidle, F.; Schimmel, J.; Blumel, N.; Galej, W. P.; Vertegaal, A. C. O.; Muller-McNicoll, M.; Zarnack, K.; Muller, S.; Dikic, I.
Show abstract
Pre-mRNA splicing is a highly regulated process orchestrated by splicing factors, cis-acting elements, and interconnected cellular processes such as transcription and chromatin remodeling. Here, we identify a novel regulatory axis involving phosphorylation and SUMOylation that governs the function of Tripartite motif-containing 28 protein (TRIM28) and its role in pre-mRNA splicing. We demonstrate that TRIM28 interacts with the spliceosomal protein USP39 in a phosphorylation-dependent manner, with non-phosphorylated TRIM28 promoting USP39 SUMOylation at defined lysine residues. This post-translational modification enhances USP39s role within the U4/U6.U5 tri-snRNP complex. Functionally, TRIM28 knockdown induces widespread alterations in alternative splicing patterns, underscoring its importance in splicing regulation. Together, our findings uncover a mechanistic link between TRIM28-mediated post-translational modifications and the modulation of spliceosomal activity, offering new insights into how splicing decisions are integrated with cellular signaling pathways.
Matching journals
The top 6 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Exon junction complex-associated multi-adapter RNPS1 nucleates splicing regulatory complexes to maintain transcriptome surveillance 94%
- SUMO conjugation regulates the activity of the Integrator complex 93%
- Distinct functions for the paralogous RBM41 and U11/U12-65K proteins in the minor spliceosome 93%
Similar papers in this journal
- Structural basis of the interaction between SETD2 methyltransferase and hnRNP L paralogs for governing co-transcriptional splicing 94%
- Intragenic recruitment of NF-κB drives alternative splicing modifications upon activation by the viral oncogene TAX of HTLV-1. 94%
- The histone methyltransferase SETD2 couples transcription and splicing by engaging pre-mRNA processing factors through its SHI domain 94%
Similar papers in this journal
- RNPS1 in PSAP complex controls periodic pre-mRNA splicing over the cell cycle 95%
- The E3 ubiquitin ligase DTX3L and the deubiquitinase USP28 fine-tune DNA double strand repair through mutual regulation of their protein levels 93%
- STING Nuclear Partners Contribute to Innate Immune Signalling Responses 92%
Similar papers in this journal
- A neuron-specific microexon ablates the novel DNA-binding function of a histone H3K4me0 reader PHF21A 94%
- SAP30BP interacts with RBM17/SPF45 to promote splicing in a subset of human short introns 93%
- A sperm-enriched 5'fragment of tRNA-Valine regulates preimplantation embryonic transcriptome and development 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.