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Intratumoral Treg ablation is sufficient to mediate tumor control systemically without autoimmunity

Bockman, A.; Gittins, B.; Zhang, C.; Hung, J.; Moreno Ayala, M.; Saddier Axe, D.; Weist, B. M.; DuPage, M.

2025-08-25 cancer biology
10.1101/2025.06.26.661765 bioRxiv
Show abstract

Regulatory T cells (Tregs) infiltrate most tumors, yet whether they suppress immune responses directly within tumor tissues is not clear. We used intratumoral (IT) delivery of diphtheria toxin (DT) in Foxp3DTR mice to deplete IT Tregs while leaving peripheral Tregs intact. IT delivery of DT reduced Treg frequencies in the tumor, which promoted potent tumor control without autoimmunity. Interestingly, this control was principally mediated by CD4+ T cells, whereas CD8+ T cells only contributed when CD4+ T cells were absent. While conventional dendritic cells (cDCs) were required to clear tumors, Batf3+ cDC1s were dispensable. Distant secondary tumors, mimicking metastases, were also controlled by IT Treg ablation. Mechanistically, IT Tregs suppressed antitumor T cell responses by blocking the acquisition of tumor antigen by cDC2s. Importantly, similar mechanisms of control were observed using a clinically translatable IT Treg-depleting anti-CCR8 antibody and reveal a distinct therapeutic strategy that leverages cDC2s and CD4+ T cells.

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