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MAPseq2: a sensitive and cost-effective barcoded connectomics method

Kim, H.; Qi, H.; Washington, C.; Liang, X.; Kebschull, J. M.

2025-08-03 neuroscience
10.1101/2025.06.23.661165 bioRxiv
Show abstract

The barcoded connectomics tool MAPseq enables highly multiplexed projection mapping of individual neurons by translating neuroanatomy into a format solvable by DNA sequencing. Here we present MAPseq2, a user-friendly protocol with up to [~]10-fold increased sensitivity and [~]10-fold decreased cost compared to the current state of the art. As MAPseq workflows are used across a range of barcoded connectomics methods, including BARseq, BRlCseq, and ConnectlD, all improvements in MAPseq2 directly transfer to these technologies.

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