Directed Evolution of a Pantoea Strain for Antibiotic Production via Interspecies Competition: A Reproducible Experimental Approach
Pavelka, J.; Janoskova, K.; Hlinkova, K.; Horova, E.; Pavelka, J.; Sima, R.
Show abstract
A novel methodology for evolving antibiotic-producing bacterial strains is presented, based on interspecies competition and environmental pressure. Using a previously undescribed strain of the genus Pantoea, we established a breeding protocol in which gradual exposure to different bacterial pathogens at increasing temperatures resulted in the selective emergence of antibiotic-producing variants. The target pathogens included Staphylococcus aureus, Acinetobacter baumannii, Streptococcus agalactiae, Escherichia coli, and Pseudomonas aeruginosa. The competitive passages were performed over a range of temperatures, gradually selecting for strains capable of inhibiting these species. Antibiotic activity was confirmed via disk diffusion assays. Although the chemical identity of the active compounds remains to be determined, their biological effects are reproducible and strain-specific. The approach provides a low-cost, scalable method for the generation of new antibiotic-producing bacterial strains.
Matching journals
The top 5 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Evaluation of bacterial proliferation with a microfluidic-based device: Antibiochip. 96%
- Mechanism of interaction of an endofungal bacterium Serratia marcescens D1 with its host and non-host fungi 96%
- Occurrence, identification and antibiogram signatures of selected Enterobacteriaceae from Tsomo and Tyhume rivers in the Eastern Cape Province, Republic of South Africa. 96%
Similar papers in this journal
- Molecular basis and evolutionary cost of a novel phenotype of macrolides/lincosamides resistance in Staphylococcus haemolyticus 96%
- Direct colorimetry of imipenem decomposition as a novel cost effective method for detecting carabapenamase producing bacteria 96%
- Honeybees exposure to veterinary drugs: how the gut microbiota is affected 95%
Similar papers in this journal
- Antimicrobial resistance determinants in silage 96%
- In vitro and in vivo efficacy of the combination of colistin and endolysins against clinical strains of Multi-Drug Resistant (MDR) pathogens 96%
- Characterization and description of Faecalibacterium butyricigenerans sp. nov. and F. longum sp. nov., isolated from human faeces 95%
Similar papers in this journal
- In vitro susceptibility to β-lactam antibiotics and viability of Neisseria gonorrhoeae strains producing plasmid-mediated broad- and extended-spectrum β-lactamases 96%
- Correlation of pathogenic factors with antimicrobial resistance of clinical Proteus mirabilis strains 96%
- Restriction of the growth and biofilm formation of ESKAPE pathogens by caprine gut-derived probiotic bacteria 95%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.