Beyond Motility: A Multiparametric Fitness Assay Framework to Evaluate Anthelmintics in Nippostrongylus brasiliensis
Anesko, K.; Midou, S.; Ruggiero-Ruff, R.; He, Y.; Jennett, J.; Nair, M. G.; Dillman, A.
Show abstract
We adapted a multiparametric fitness assay panel--including ATP quantification, larval growth, pigmentation, and cuticle permeability--to assess Nippostrongylus brasiliensis viability in vitro. To validate this system, we benchmarked two known anthelmintics with distinct modes of action: quinidine, a heme detoxification disruptor, and pyrantel pamoate, a neuromuscular antagonist. We then applied this framework to NCR247, a plant-derived heme-sequestering peptide with proposed antiparasitic activity. While the assay panel sensitively detected physiological disruption by known anthelmintics, NCR247 did not significantly impair parasite fitness across any tested parameter. These findings highlight the utility of integrated viability assays and suggest further in vivo or delivery-optimized studies may be necessary to evaluate NCR247 efficacy.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- De novo identification of toxicants that cause irreparable damage to parasitic nematode intestinal cells 96%
- Induced pluripotent stem cell-derived human macrophages as an infection model for Trypanosoma cruzi 94%
- JQ-1 ameliorates schistosomiasis liver granuloma in mice by suppressing male and female reproductive systems and egg development of Schistosoma japonicum 94%
Similar papers in this journal
Similar papers in this journal
Similar papers in this journal
Similar papers in this journal
- Screening the Medicines for Malaria Venture Pathogen Box for invasion and egress inhibitors of the blood stage of Plasmodium falciparum reveals several inhibitory compounds 94%
- The transmission-blocking effects antimalarial drugs revisited: mosquito fitness costs and sporontocidal effects of artesunate and sulfadoxine-pyrimethamine 93%
- Transgenic pyrimethamine-resistant P. falciparum reveals transmission blocking potency of P218, a novel antifolate 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.