The assembly of the membrane arm of mitochondrial complex I follows evolutionarily distinct routes
Parlar, S.; Marofke, M.; Kühn, K.; Gawryluk, R.; Meyer, E.
Show abstract
Complex I is the largest enzyme of the mitochondrial oxidative phosphorylation system. It is composed of core subunits that are conserved across bacteria and eukaryotes and accessory subunits that are lineage-specific. Consequently, the assembly of complex I likely requires lineage-specific assembly machineries. Complex I assembly has been mainly studied in mammals. It follows a modular pathway in which modules are assembled independently and then associated together to form the mature complex. Here, we identify and characterize PAF1 as an assembly factor for the membrane arm of complex I in plants. We show that this protein plays a role in stabilizing a module of the membrane arm prior to its association with the rest of the complex. PAF1 was likely present in the last eukaryotic common ancestor and has been lost in mammals in which the assembly of the membrane arm involves assembly factors not conserved in plants. Altogether, we provide molecular evidence that the assembly of the membrane arm of complex I can be performed by two different machineries.
Matching journals
The top 9 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Mpf1 is a novel factor that affects the dual distribution of tail-anchored proteins between mitochondria and peroxisomes 95%
- The ER-SURF pathway uses ER-mitochondria contact sites for protein targeting to mitochondria 95%
- A quantitative fluorescence-based approach to study mitochondrial protein import 94%
Similar papers in this journal
- Correction of frameshift mutations in the atpB gene by translational recoding in chloroplasts of Oenothera and tobacco. 94%
- Proteomic studies of the Arabidopsis TRAPP complexes reveal conserved organization and a novel plant-specific component with a role in plant development 94%
- The mitochondrial pyruvate carrier (MPC) complex is one of three pyruvate-supplying pathways that sustain Arabidopsis respiratory metabolism 94%
Similar papers in this journal
- A systematic bi-genomic split-GFP assay illuminates the mitochondrial matrix proteome and protein targeting routes 95%
- Acidocalcisome-like vacuoles constitute a feedback-controlled phosphate buffering system for the cytosol 94%
- The Arabidopsis V-ATPase is localized to the TGN/EE via a seed plant specific motif and acts in a partially redundant manner with the tonoplast enzyme 94%
Similar papers in this journal
- Nucleo-plastidic PAP8/pTAC6 couples chloroplast formation with photomorphogenesis 94%
- Proteomic identification of the interactome of stalled ribosome nascent chain complexes translating the thylakoid membrane protein D1 94%
- Increased levels of the mitochondrial import factor Mia40 prevent the aggregation of polyQ proteins in the cytosol 93%
Similar papers in this journal
- FRIENDLY (FMT) is an RNA binding protein associated with cytosolic ribosomes at the mitochondrial surface 95%
- Mitochondrial ATP Synthase Subunit d, a Component of the Peripheral Stalk, is Essential for Growth and Heat Stress Tolerance in Arabidopsis thaliana. 95%
- PAM18-3, a J-domain protein, maintains mitochondrial integrity and plant growth and development in Arabidopsis thaliana 94%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.