Infrared Laser Sampling of Low Volumes Reveals Marker Lipids in Palatine Tonsil Carcinoma via Shotgun Lipidomics
Kerkhoff, L.; Moritz, M.; Eggert, D.; Worthmann, A.; Heeren, J.; Zech, H.; Clauditz, T.; Wilczak, W.; Schlueter, H.; Betz, C. S.; Bottcher, A.; Hahn, J.
Show abstract
Complete surgical resection is essential for oropharyngeal squamous cell carcinoma (OPSCC) therapy, underscoring the need for improved intraoperative margin assessment. To advance in-vivo diagnostics of OPSCC, Nanosecond infrared laser (NIRL) tissue sampling combined with shotgun lipidomic analysis reveals lipidome differences between OPSCC tissue and adjacent healthy tissue. Ablations were performed on tonsil squamous cell carcinoma on n=28 samples from 11 patients with an established chamber setup and a subset of n=6 samples from three patients with a custom-made handheld applicator. Welchs t-test results (p=0.05, two-fold change) revealed a similar OPSCC lipid profile in 7 of 11 patients. Potential tumor lipid markers were identified as consistently significantly increased, despite biological heterogeneity of the samples, underscoring their potential diagnostic value. Tissue ablation with a custom-made handheld applicator coupled to a laser fiber was successful and the lipidomic analysis was consistent to the chamber setup. Although our setup is currently limited by an ablation time exceeding one minute, this study demonstrates the potential of the handheld applicator for future applications such as endoscopy or intraoperative diagnostics.
Matching journals
The top 13 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Spatial omics imaging of fresh-frozen tissue and routine FFPE histopathology on a single cancer needle core biopsy: freezing device and multimodal workflow 93%
- Automated Raman micro-spectroscopy of epithelial cells for the high-throughput classification. 93%
- Altered plasma, urine, and tissue profiles of sulfatides and sphingomyelins in patients with renal cell carcinoma 93%
Similar papers in this journal
- Rapid Screening of COVID-19 Disease Directly from Clinical Nasopharyngeal Swabs using the MasSpec Pen Technology 94%
- Heat n Beat: A universal high-throughput end-to-end proteomics sample processing platform in under an hour 93%
- UCL-MetIsoLib: A Public High-Resolution Tandem Mass Spectrometry Library for HILIC-Based Isomer-Resolved Profiling of Glycolysis, Central Carbon Metabolism, and Beyond in Urine, Plasma, Tissues, Cells, and Patient-Derived Organoids 93%
Similar papers in this journal
- Vibrational Spectroscopy Identifies Myocardial Chemical Modifications in Heart Failure with Preserved Ejection Fraction 92%
- Panomics reveals patient-individuality as the major driver for colorectal cancer progression 92%
- Deep Learning-Based 3D Leukocyte Differentiation Using Label-Free Higher Harmonic Generation Microscopy 91%
Similar papers in this journal
- Untargeted lipidomics of non-small cell lung carcinoma shows differentially abundant lipid classes in cancer vs non-cancer tissue 94%
- Matrix selection for the visualization of small molecules and lipids in brain tumors using untargeted MALDI-TOF mass spectrometry imaging 93%
- Utility of an untargeted metabolomics approach using a 2D GC-GC-MS platform to distinguish relapsing and progressive multiple sclerosis 92%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.