Complex rheology of condensin in entangled DNA
Conforto, F.; Valdes, A.; Vanderlinden, W.; Michieletto, D.
Show abstract
Structural-Maintenance-of-Chromosome (SMC) complexes such as condensins are well-known to dictate the folding and entanglement of interphase and mitotic chromosomes. However, their role in modulating the rheology and viscoelasticity of entangled DNA is not fully understood. In this work, we discover that physiological concentrations of yeast condensin increase both the effective viscosity and elasticity of dense solutions of{lambda} -DNA even in absence of ATP. By combining biochemical assays and single-molecule imaging, we discover that yeast condensin can proficiently bind double-stranded DNA through its hinge domain, in addition to its heads. We further discover that presence of ATP fluidifies the entangled solution possibly by activating loop extrusion. Finally, we show that the observed rheology can be understood by modelling SMCs as transient crosslinkers in bottle-brush-like entangled polymers. Our findings help us to understand how SMCs affect the rheology and dynamics of the genome.
Matching journals
The top 3 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Microscopic interactions control a structural transition in active mixtures of microtubules and molecular motors 96%
- Multiscale mechanics and temporal evolution of vimentin intermediate filament networks 96%
- Insulation between adjacent TADs is controlled by the width of their boundaries through distinct mechanisms 96%
Similar papers in this journal
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.