A Single-cell and Spatially Resolved Cell Atlas of Human Esophageal Squamous Cell Carcinoma
shi, y.; An, K.; Qi, Y.; Zhang, X.; Wang, Y.; Zhang, X.; Zhou, S.; Li, O.; Zhou, J.; Du, Y.; Hou, M.; Yang, Y.-G.; Kan, Q.; Tian, X.
Show abstract
Tumor heterogeneity and the suppressive microenvironment are key challenges that limit the effectiveness of cancer treatment. In this study, we systematically elucidated the molecular characteristics and mechanisms underlying the suppressive immune microenvironment via a combination of single-cell RNA sequencing, spatial transcriptomics, and metabolomics for a series of human esophageal squamous cell carcinoma (ESCC) and matched nontumor tissues. We found that COL17A1+ epithelial cells presented greater malignancy, characterized by triglyceride (TG) and phosphocholine (PC) accumulation. We also identified a tumor-specific POSTN+ fibroblast subgroup. We found a unique epithelial-fibroblast niche with low infiltration of effector immune cells and substantial enrichment of lipids, composed of POSTN+ fibroblasts and COL17A1+ epithelial cells, where their crosstalk contributed to tumor progression. We confirmed that the INHBA/TP63 axis plays a key role in mediating the regulation of COL17A1+ tumor cells by POSTN+ fibroblasts. Our findings provided new insights into the characteristics of the tumor microenvironment and the crosstalk between tumor and fibroblasts, offering valuable multiomics data resources for elucidating tumor progression mechanisms.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Unveiling chemotherapy-induced immune landscape remodeling and metabolic reprogramming in lung adenocarcinoma by scRNA-sequencing 97%
- S100a4+ alveolar macrophages accelerate the progression of precancerous atypical adenomatous hyperplasia by promoting the angiogenic function regulated by fatty acid metabolism 96%
- Comprehensive characterization of tumor microenvironment in colorectal cancer via histopathology-molecular analysis 96%
Similar papers in this journal
Similar papers in this journal
- m6A Demethylase FTO Stabilizes LINK-A to Exert Oncogenic Roles via MCM3-Mediated Cell Cycle Progression and HIF-1α Activation 96%
- P-cadherin mechanoactivates tumor- mesothelium metabolic coupling to promote ovarian cancer metastasis 95%
- MAPK14/p38α Shapes the Molecular Landscape of Endometrial Cancer and promotes Tumorigenic Characteristics 95%
Similar papers in this journal
- Comprehensive Analysis of Spatial Architecture in Primary Liver Cancer 96%
- Immunotherapy of glioblastoma explants induces interferon-γ responses and immune cell rearrangements in tumor center, but not periphery 95%
- Selective targeting of TBXT with DARPins identifies regulatory networks and therapeutic vulnerabilities in chordoma 95%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.