A Cell Type-Specific Role for Tubb6 in Ciliogenesis of Xenopus Epidermal Multiciliated Cells
Xu, X.; Ross, J.; Clark, F.; Wei, S.; Sun, J.
Show abstract
Cilia are microtubule-based organelles found on the surface of most eukaryotic cells. These microtubules are composed of - and {beta}-tubulin heterodimers, and different tubulin isotypes can confer distinct properties to microtubules. Despite their importance, the contribution of individual tubulin isotype to cilia formation and function remains largely unexplored in vertebrates. Here, we identify a critical role for the {beta}-tubulin isotype Tubb6 in the formation of motile cilia in Xenopus epidermal multiciliated cells (MCCs). Tubb6 mRNA is selectively expressed in MCCs, and its protein product localizes to ciliary axonemes. Loss of Tubb6 leads to a marked reduction in cilia number and length, resulting in defective MCC function. In contrast, mono-motile cilia in the gastrocoel roof plate are unaffected by Tubb6 depletion, suggesting a selective requirement for ciliogenesis in MCCs. Together, our findings uncover a cell type-specific role for Tubb6 in motile cilia formation and highlight the functional specialization of tubulin isotypes in vertebrate cilia assembly.
Matching journals
The top 5 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Collective nuclear behavior shapes bilateral nuclear symmetry for subsequent left-right asymmetric morphogenesis in Drosophila 95%
- Nascent dendrite branches initiated by a localized burst of Spire-dependent actin polymerization. 94%
- Neural tube closure requires the endocytic receptor Lrp2 and its functional interaction with intracellular scaffolds 94%
Similar papers in this journal
- Brain development mutations in the β-tubulin TUBB result in defective ciliogenesis 96%
- Glia detect and mount a protective response to loss of dendrite substructure integrity in C. elegans 95%
- A release-and-capture mechanism generates an essential non-centrosomal microtubule array during tube budding 94%
Similar papers in this journal
- GJA1 Depletion Causes Ciliary Defects by Affecting Rab11 Trafficking to the Ciliary Base 95%
- Female-germline specific protein Sakura interacts with Otu and is crucial for germline stem cell renewal and differentiation and oogenesis 95%
- Ectosome uptake by glia sculpts Caenorhabditis elegans cilia 94%
Similar papers in this journal
- DYF-5 regulates intraflagellar transport by affecting train turnaround 94%
- A large reverse-genetic screen identifies numerous regulators of testis nascent myotube collective cell migration and collective organ sculpting 93%
- The ARF GAPs ELMOD1 and ELMOD3 act at the Golgi and Cilia to Regulate Ciliogenesis and Ciliary Protein Traffic 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.