Drone methods and educational resources for plant science and agriculture

Technological advances have made drones (UAVs) increasingly important tools for the collection of trait data in plant science. Many costs for the analysis of plant populations have dropped precipitously in recent decades, particularly for genetic sequencing. Similarly, hardware advances have made it increasingly simple and practical to capture drone imagery of plant populations.However, converting this imagery into high-precision and high-throughput tabular data has become a major bottleneck in plant science. Here, we describe high-throughput phenotyping methods for the analysis of numerous plant traits based on imagery from diverse sensor types. Methods can be flexibly combined to extract data related to canopy temperature, area, height, volume, vegetation indices, and summary statistics derived from complex segmentations and classifications. We then describe educational and training resources for these methods, including a web page (PlantScienceDroneMethods.github.io) and an educational YouTube channel (https://www.youtube.com/@travisparkerplantscience) with step-by-step protocols, example data, and example scripts for the whole drone data processing pipeline. These resources facilitate the extraction of high-throughput and high-precision phenomic data, removing barriers to the phenomic analysis of large plant populations.