sKL/mKL Transcript Ratio and Protein Localization Define a Species- and Region-Specific Klotho Signature in the CNS and AD Progression
Blanch, R.; Esandi, J.; Guerrero-Yagüe, R.; Del Rey, J.; Roig-Soriano, J.; Lleo, A.; Carro, E.; Mestre-Frances, N.; Devau, G.; Ferrer, I.; Meseguer, A.; Lanciego, J. L.; Almolda, B.; Bosch, A.; Chillon, M.
Show abstract
-Klotho is a multifunctional protein widely recognized for its anti-aging and neuroprotective properties. This study investigates the expression and localization of the secreted Klotho (s-KL) isoform in the human brain and its potential role in Alzheimers disease. Using RT-qPCR, we observed that the s-KL transcript predominates over the membrane-bound KL (m-KL) in multiple brain regions, a pattern consistent in macaques and lemurs. Immunohistochemistry and immunoprecipitation assays confirmed the presence of the s-KL protein in human and mouse brain parenchyma, revealing species-specific cellular localization. In human cerebrospinal fluid (CSF), s-KL constitutes [~]28% of total KL, with levels significantly reduced in mild dementia-AD patients. These findings underscore s-KLs potential neuroprotective role and highlight its differential regulation and expression during AD progression.
Matching journals
The top 10 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Protein farnesylation is upregulated in Alzheimer's human brains and neuron-specific suppression of farnesyltransferase mitigates pathogenic processes in Alzheimer's model mice 96%
- Cathepsin B abundance, activity and microglial localisation in Alzheimer's disease-Down syndrome and early onset Alzheimer's disease; the role of elevated cystatin B 96%
- Proteomic analysis across patient iPSC-based models and human post-mortem hippocampal tissue reveals early cellular dysfunction, progression, and prion-like spread of Alzheimer s disease pathogenesis 95%
Similar papers in this journal
- Apolipoprotein E abundance is elevated in the brains of individuals with Down syndrome-Alzheimer's disease 96%
- The Olfactory Epithelium: A Critical Gateway for Pathological Tau Propagation and a Target for Mitigating Tauopathy in the Central Nervous System 95%
- Transmembrane protein 97 is a potential synaptic amyloid beta receptor in human Alzheimer's disease 95%
Similar papers in this journal
- Diabetic phenotype in mouse and humans with β-amyloid pathology reduces the number of microglia around β-amyloid plaques 96%
- BIN1 protein isoforms are differentially expressed in astrocytes, neurons, and microglia: neuronal and astrocyte BIN1 implicated in Tau pathology 95%
- Genetic perturbations of disease risk genes in mice capture transcriptomic signatures of late-onset Alzheimer’s disease 94%
Similar papers in this journal
- Modeling human age-associated increase in Gadd45γ expression leads to spatial recognition memory impairments in young adult mice 93%
- Mitochondrial pathway polygenic risk scores are associated with Alzheimer's Disease 93%
- Age-dependent dysregulation of locus coeruleus firing in a transgenic rat model of Alzheimers disease 93%
Similar papers in this journal
- Antagonists targeting eEF2 kinase rescue multiple aspects of pathophysiology in Alzheimer's disease model mice 94%
- Suppression of amyloid-β secretion from neurons by cis-9, trans-11-octadecadienoic acid, an isomer of conjugated linoleic acid 94%
- Physiopathological changes of ferritin mRNA density and distribution in hippocampal astrocytes in the mouse brain 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.