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Cholinergic blockade reveals role for human hippocampal theta in encoding but not retrieval

Gedankien, T.; Kriegel, J.; Zabeh, E.; McDonagh, D.; Lega, B.; Jacobs, J.

2025-05-13 neuroscience
10.1101/2025.05.12.653487 bioRxiv
Show abstract

Cholinergic dysfunction is a hallmark of Alzheimers disease and other memory disorders. Yet, the neurophysiological mechanisms linking cholinergic signaling to memory remain poorly understood. In this study, we administered scopolamine, a muscarinic cholinergic antagonist, to neurosurgical patients with intracranial electrodes as they performed an associative recognition memory task. When scopolamine was present at encoding, we observed disruptions to hippocampal slow theta oscillations (2-4 Hz), with selective impairments to recollection-based memory. However, when scopolamine was present during retrieval alone, we observed disruptions to slow theta without impaired memory performance. These disruptions included dose-dependent reductions in theta power, theta phase reset, and encoding-retrieval pattern reinstatement. Together, our results challenge the notion that theta oscillations are necessary for memory retrieval, and instead suggest that theta universally reflects an encoding-related neural state. These findings motivate updates to current models of acetylcholines role in memory and may inform future therapies targeting rhythmic biomarkers of memory dysfunction.

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