Robust TCR production for the structural study of TCR-pMHC complexes
Oyon-Olea, L.; Dichiara-Rodriguez, M. G.; Hervas-Stubbs, S.; Erausquin, E.; Lopez-Sagaseta, J.
Show abstract
A precise comprehension of how T cell receptors (TCRs) engage their antigens is pivotal for advancing basic research and T cell immunotherapy in cancer. While TCR refolding from inclusion bodies has greatly facilitated X-ray studies over the past decades, the procedures remain labor-intensive and can yield poorly. We have developed a simplified strategy for efficient production of soluble TCRs in CHO cells which, coupled with the removal of N-glycosylation, enable structural studies of TCR-pMHC complexes. An equivalent of just 20 ml of cell culture delivered sufficient deglycosylated TCR (dgTCR) to screen, upon complexation with a cognate pMHC, over 350 crystallization conditions and obtain a high-resolution dataset. This approach illustrates an effective alternative for TCR production to support studies devoted to research and development of TCRs.
Matching journals
The top 7 journals account for 50% of the predicted probability mass.
Similar papers in this journal
Similar papers in this journal
Similar papers in this journal
- AlphaFold accurately predicts distinct conformations based on oligomeric state of a de novo designed protein 94%
- The role of evolutionarily metastable oligomeric states in the optimization of catalytic activity 93%
- Structure of Human BCCIP and Implications for Binding and Modification of Partner Proteins 93%
Similar papers in this journal
- Vaccine-elicited and naturally elicited antibodies differ in their recognition of the HIV-1 fusion peptide 93%
- In silico design of Phl p 6 variants with altered folding stability significantly impacts antigen processing, immunogenicity and immune polarization 92%
- Systematic computer-aided disulfide design as a general strategy to stabilize prefusion class I fusion proteins 92%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.