A conserved triple arginine motif in OMA-1 is required for RNA-binding activity and embryo viability
Ertekin, A.; Noronha, S. T.; Darko, C.; Massi, F.; Ryder, S. P.
Show abstract
Sexually reproducing organisms make haploid gametes--oocytes and spermatocytes--that combine during fertilization to make an embryo. While both gametes contain similar DNA content, oocytes contain the bulk of the cytoplasm including maternally supplied mRNAs and proteins required prior to zygotic gene activation. RNA-binding proteins are key regulators of these maternal transcripts. In Caenorhabditis elegans, the tandem zinc finger proteins OMA-1 and OMA-2 are required for fertilization. Here, we show that OMA-1 RNA-binding activity requires a short basic region immediately up-stream from the canonical tandem zinc finger domain. Mutation of this region in animals produces a phenotype distinct from a genetic null. Oocytes can be fertilized, but fail to form an intact chitin egg-shell, frequently break in utero, and arrest prior to morphogenesis. Our results identify a critical region outside of the canonical RNA-binding domain required for both RNA-binding activity as well as revealing a new role for OMA-1 during the oocyte-to-embryo transition.
Matching journals
The top 5 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Functionally non-redundant paralogs spe-47 and spe-50 encode FB-MO associated proteins and interact with him-8 96%
- Exploring the evolution and function of Canoes intrinsically disordered region in linking cell-cell junctions to the cytoskeleton during embryonic morphogenesis 96%
- The evolutionarily conserved PhLP3 is essential for sperm development in Drosophila melanogaster 95%
Similar papers in this journal
- The Chromatin Remodeling Protein CHD-1 and the EFL-1/DPL-1 Transcription Factor Cooperatively Down Regulate CDK-2 to Control SAS-6 Levels and Centriole Number 96%
- A Genetic Screen for Suppressors of Cryptic 5' Splicing in C. elegans Reveals Roles for KIN17 and PRCC in Maintaining Both 5' and 3' Splice Site Identity 96%
- Systematic mapping of rRNA 2'-O methylation during frog development and involvement of the methyltransferase Fibrillarin in eye and craniofacial development in Xenopus laevis 96%
Similar papers in this journal
Similar papers in this journal
- GNL3 is an evolutionarily-conserved stem cell gene influencing cell proliferation, animal growth, and regeneration in the hydrozoan Hydractinia 94%
- The seminal vesicle is a juvenile hormone-responsive tissue in adult male Drosophila melanogaster 94%
- Kre28-Spc105 interaction is essential for Spc105 loading at the kinetochore. 93%
Similar papers in this journal
- Drosophila architectural protein CTCF is not essential for fly survival and is able to function independently of CP190 95%
- The SAGA HAT module is tethered by its SWIRM domain and modulates activity of the SAGA DUB module 92%
- Evidence of target-mediated miRNA degradation in Drosophila ovarian cell culture 92%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.