Back

Adhesion-derived condensates control component availability to regulate adhesion dynamics

Dibus, M.; Enkavi, G.; Chastney, M.; Vattulainen, I.; Ivaska, J.

2025-05-09 cell biology
10.1101/2025.05.08.652869 bioRxiv
Show abstract

Integrin adhesion complexes mediate cell-extracellular matrix (ECM) interactions and undergo dynamic remodelling to regulate cell adhesion and migration. Here, we demonstrate that tensin 1 (TNS1), a multidomain adhesion adaptor protein linking active integrins with the actin cytoskeleton, undergoes phase separation in cells. Endogenous TNS1 condensates are formed upon focal adhesion disassembly or limited integrin-ECM engagement in both 2D and 3D environments, acting as reservoirs for inactive adhesion proteins. Combining functional experimental approaches with phosphoproteomics, we identify the TNS1 intrinsically disordered region as the main driver of TNS1 condensation and demonstrate a negative regulatory role of phosphorylation on condensate assembly upon activation of stress-responsive kinases. Finally, we confirm the functional effects of phosphorylation-dependent TNS1 condensation on adhesion dynamics and cell migration. Together, our findings highlight TNS1 condensation as a regulatory mechanism controlling local availability of inactive adhesion proteins, with direct implications on adhesion dynamics and cell behaviour.

Published in Nature Communications (predicted rank #1) · training set

Matching journals

The top 5 journals account for 50% of the predicted probability mass.

Nature Communications · published here
○ 5641 papers in training set
Top 5%
25.7%
Similar papers in this journal
2
Journal of Cell Biology
● 392 papers in training set
Top 0.7%
7.6%
Similar papers in this journal
3
Nature Cell Biology
○ 118 papers in training set
Top 0.2%
7.6%
Similar papers in this journal
4
Cell Reports
○ 1498 papers in training set
Top 4%
7.0%
Similar papers in this journal
5
eLife
● 5828 papers in training set
Top 20%
6.1%
Similar papers in this journal
50% of probability mass above
6
The EMBO Journal
○ 309 papers in training set
Top 0.6%
5.4%
7
Molecular Cell
○ 350 papers in training set
Top 1%
5.0%
8
Science Advances
● 1243 papers in training set
Top 9%
3.9%
9
iScience
○ 1154 papers in training set
Top 8%
3.1%
10
Proceedings of the National Academy of Sciences
● 2444 papers in training set
Top 19%
2.7%
11
Nature Chemical Biology
○ 119 papers in training set
Top 1%
2.3%
12
Cell
○ 431 papers in training set
Top 5%
2.1%
13
Communications Biology
○ 993 papers in training set
Top 13%
1.8%
14
EMBO Reports
○ 263 papers in training set
Top 4%
1.7%
15
Advanced Science
○ 286 papers in training set
Top 5%
1.7%
16
Current Biology
○ 665 papers in training set
Top 7%
1.5%
17
Nature Materials
○ 28 papers in training set
Top 0.3%
1.4%
18
Nature Structural & Molecular Biology
236 papers in training set
Top 3%
1.1%
19
Life Science Alliance
● 285 papers in training set
Top 6%
1.0%
20
Molecular Systems Biology
○ 162 papers in training set
Top 3%
0.8%
21
Developmental Cell
○ 196 papers in training set
Top 4%
0.8%
22
Journal of Cell Science
● 393 papers in training set
Top 5%
0.8%
23
Scientific Reports
○ 3612 papers in training set
Top 74%
0.8%
24
Molecular Biology of the Cell
● 311 papers in training set
Top 4%
0.6%

"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.