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Developmental landscape of shark telencephalon sheds light on the evolution of telencephalic cell types and the ancient origin of Cajal-Retzius cells

Quintana-Urzainqui, I.; Oel, P.; Berger, T.; Pan, L.; Papadopoulos, N.; Serka, G.; Verbanac, A.; Boersig, M.; Torres-Sabino, D.; Rollan-Delgado, I. C.; Santangeli, L.; Kaessmann, H.; Arendt, D.

2025-05-08 developmental biology
10.1101/2025.05.08.652169 bioRxiv
Show abstract

The emergence of predation and associated complex behaviors in jawed vertebrates (gnathostomes) were major driving forces in brain evolution. To gain insight into the neuronal complexity of the last common gnathostome ancestor, we studied the development of the shark telencephalon by combining single-cell and spatial transcriptomics. Our findings suggest an ancient origin of pallial neurogenesis, including basal progenitors, which were previously only identified in tetrapods. We find evidence for migrating gabaergic neurons populating the dorsal pallium, where we observe incipient layering. Finally, we provide evidence for the existence of Cajal-Retzius cells in the developing shark telencephalon, with a conserved molecular profile and tissue localization relative to mammals. Our analyses revealed that major features of the vertebrate pallium arose much earlier than previously thought, in the gnathostome lineage.

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