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mtgRNA-db: An annotated database of multi-target CRISPR-Cas9 guide-RNAs in the human genome

Modrek, A. S.; Huynh, K.; Do, C.; Karp, J.; Deng, Y.; Zhang, Z.; Ezhilarasan, R.; Valor, B.; Cova, G.; Skok, J. A.; Sulman, E. P.

2025-05-10 bioinformatics
10.1101/2025.05.07.652656 bioRxiv
Show abstract

CRISPR-Cas9 guide-RNA design tools can be used to identify guide-RNAs that target single human genomic loci. However, these approaches limit their effect to a single locus. Here, we generate a database of potential individual guide-RNAs that target multiple sites in the genome at once. All guide-RNAs in this database are curated with on- and off-target quantification and enrichment scores for genomic elements such as gene elements, regulatory elements, repetitive elements, and transcription factor motifs. This tool enables rationale mass targeting of genomic loci with a single guide for functional studies. We created a web-app for user-friendly guide-RNA selection (https://modreklab.shinyapps.io/guiderna/).

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"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.