Detecting Protein Higher-Order Structural Changes Using Kinase as a Phospho-Labeler

We developed an approach to detect protein conformational changes on a proteome-wide scale by labeling proteins with phosphate groups through in vitro kinase reactions. This structural proteomics approach combines residue-specific labeling based on substrate recognition by protein kinases with quantitative phosphoproteomics using phosphopeptide enrichment, enabling proteome-wide and site-specific profiling of protein conformational changes. By performing in vitro phosphorylation of myoglobin with and without heat denaturation and of HEK293T cell extracts with and without protease treatment, we demonstrated that differences in substrate protein structure are reflected in in vitro phosphorylation efficiency. Moreover, we successfully identified protein conformational changes upon RNA digestion in non-denatured cell extract proteome samples on a proteome-wide scale with high sensitivity by comparing phosphorylation efficiencies. This approach enables residue-specific readout of structural dynamics within the intracellular proteome. Graphical abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=119 SRC="FIGDIR/small/652599v1_ufig1.gif" ALT="Figure 1"> View larger version (30K): org.highwire.dtl.DTLVardef@b2c6c8org.highwire.dtl.DTLVardef@118b159org.highwire.dtl.DTLVardef@2374adorg.highwire.dtl.DTLVardef@1c14880_HPS_FORMAT_FIGEXP M_FIG C_FIG MotivationMass spectrometry (MS)-based structural analysis of proteins often involves labeling of exposed amino acid residues, followed by digestion and identification of labeled peptides by MS. However, the heterogeneity of labeled peptides and the complexity of biological samples hinder proteome-wide analysis of protein higher-order structures. To address this issue, we devised an approach that utilizes site-specific labeling with phosphate groups, taking advantage of the substrate recognition provided by protein kinases. The phosphate groups serve as enrichment handles, enabling selective extraction and highly sensitive analysis of labeled sites. HighlightsO_LIProtein labeling with phosphate groups using in vitro kinase reaction C_LIO_LIQuantitative MS-based readout of in vitro phosphorylation efficiency C_LIO_LIIn vitro phosphorylation efficiency reflects protein conformational changes C_LIO_LIProteome-wide and site-specific identification of protein conformational changes C_LI