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Powerful one-dimensional scan to detect heterotic QTL

Li, G.; Schmidt, R. H.; Zhao, Y.; Reif, J. C.; Jiang, Y.

2025-05-12 genetics
10.1101/2025.05.06.652159 bioRxiv
Show abstract

To meet the growing global demand for food, increasing yields through heterosis in agriculture is crucial. A deep understanding of the genetic basis of heterosis has led to the development of a quantitative genetic framework that incorporates both dominance and epistatic effects. However, incorporating all pairwise epistatic interactions is computationally challenging due to the large sequencing depth and population sizes needed to uncover the genes behind complex traits. In this study, we developed hQTL-ODS, a one-dimensional scanning method that directly assesses the net contribution of each locus to heterosis. Simulations show that hQTL-ODS reduces computational time while offering higher power and lower false-positive rate. We applied this method to a population of 5,234 wheat hybrids with whole-genome sequenced profile, revealing key epistatic hubs that play a critical role in determining heterosis. Our findings offered valuable insights for improving breeding strategies to enhance crop yields.

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