The LOTUS domain of Oskar promotes localisation of both protein and mRNA components of Drosophila germ plasm
Repouliou, A.; Srouji, J. R.; Rivard, E. L.; Leschziner, A.; Extavour, C. G.
Show abstract
Germ cells transmit genetic information to the next generation in multicellular organisms. In Drosophila melanogaster, germ cells are determined by germ plasm, a specialised cytoplasm assembled by the Oskar protein. The current view of the molecular mechanism of germ plasm assembly attributes recruitment of protein and mRNA germ plasm components to distinct domains of the Oskar protein, called the LOTUS and OSK domains respectively. However, most evidence for this model is based on in vitro studies. Here we test the ability of Oskar variants to assemble functional germ plasm in vivo. We found that Vasa recruitment was largely unperturbed by LOTUS deletion or mutations in vivo. In contrast, nanos and pgc mRNA recruitment was affected by LOTUS domain perturbations, despite the current model attributing mRNA recruitment to the distinct OSK domain. Taken together, these data suggest a revision of the prevailing modular view of Oskars structure-function mechanism.
Matching journals
The top 2 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- A DEAD-box helicase drives the partitioning of a pro-differentiation NAB protein into nuclear foci 97%
- Bicc1 and dicer regulate left-right patterning through posttranscriptionalcontrol of the Nodal-inhibitor dand5 96%
- Germ granule association drives small RNA specificity for a nuclear Argonaute protein 96%
Similar papers in this journal
- An Axon-Pathfinding Mechanism Preserves Epithelial Tissue Integrity 97%
- Evolutionary diversification reveals distinct somatic versus germline cytoskeletal functions of the Arp2 branched actin nucleator protein 96%
- The Balbiani body is formed by microtubule-controlled molecular condensation of Buc in early oogenesis 96%
Similar papers in this journal
Similar papers in this journal
- Non-autonomous regulation of germline stem cell proliferation bysomatic MPK-1/MAPK activity in C. elegans 96%
- Integrated Stress Response signaling acts as a metabolic sensor in fat tissues to regulate oocyte maturation and ovulation 96%
- The Intrinsically Disordered Region of Coronins Fine-tunes Oligomerization and Actin Polymerization 96%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.