Modeling tissue-resident macrophage development from mouse pluripotent stem cells
Baako, A. K.; Vijayakumar, R.; Medina-Cano, D.; Wang, Z.; Romero-Pichardo, J.; Fadojutimi, K.; Do, S. C.; Lin, Y.; Islam, M. T.; Dixit, S.; Trzeciak, A.; Perry, J. S. A.; Vierbuchen, T.
Show abstract
Tissue-resident macrophages (TRMs) are innate immune cells that participate in tissue development, homeostasis, and immune surveillance. Extensive efforts have been made to recapitulate TRM development from pluripotent stem cells (PSCs) in vitro to study molecular and cellular mechanisms of TRM development and to create cellular models of disease. However, available PSC models of mouse TRM development exhibit low overall efficiencies of TRM generation, produce heterogeneous off-target populations, and rely upon undefined media components, thus limiting their reproducibility, scalability, and widespread application as an experimental platform for TRM biology. To address these important limitations, we developed an efficient and reproducible protocol to faithfully recapitulate the stepwise differentiation of mouse PSCs (epiblast stem cells) into unspecialized, proliferative TRMs through the pro-definitive hematopoietic program under defined conditions. These immature TRMs can stably integrate into developing mouse neural organoids in vitro and acquire features of microglia. In addition, PSC-derived immature TRMs can stably engraft into the lung niche in vivo and adopt alveolar macrophage characteristics. This new platform for modeling mouse TRM development represents a powerful experimental model system for studying TRM function and dysfunction in development and disease.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Developmental regulation of endothelial-to-hematopoietic transition from induced pluripotent stem cells 98%
- Tropomyosin 1 deficiency facilitates cell state transitions and enhances hemogenic endothelial cell specification during hematopoiesis 97%
- High resolution multi-scale profiling of embryonic germ cell-like cells derivation reveals pluripotent state transitions in humans 96%
Similar papers in this journal
- A single cell framework identifies functionally and molecularly distinct multipotent progenitors in adult human hematopoiesis 96%
- Chromatin state barriers enforce an irreversible mammalian cell fate decision 95%
- Multipotent progenitors and hematopoietic stem cells arise independently during the endothelial to hematopoietic transition in the early mouse embryo 95%
Similar papers in this journal
- Post-Transplant Administration of G-CSF Impedes Engraftment of Gene Edited Human Hematopoietic Stem Cells by Exacerbating the p53-Mediated DNA Damage Response 96%
- Resolving fate and transcriptome of hematopoietic stem cell clones 95%
- Generation of human alveolar epithelial type I cells from pluripotent stem cells 94%
Similar papers in this journal
- A mouse organoid platform for modeling cerebral cortex development and cis-regulatory evolution in vitro 94%
- Sex biased human thymic architecture guides T cell development through spatially defined niches 94%
- Differentiation of human intestinal organoids with endogenous vascular endothelial cells 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.