CoreTIA: a modular, statistically robust transduction inhibition assay for AAV neutralization
Kovacs, B.; Somogyi, F.; Szabo, V.; Nagy, Z. Z.; Hernadi, I.; Matyas, F.; Vanduffel, W.; Szemlaky, Z.; Rozsa, B.; Ulbert, I.; Hillier, D.
Show abstract
Adeno-associated virus (AAV) gene therapy is often limited by pre-existing neutralizing antibodies (NAbs), yet current assays for NAb detection lack standardization and rarely quantify uncertainty, complicating cross-study comparisons. We present coreTIA (core Transduction Inhibition Assay), a modular experimental protocol combined with a statistically robust analysis pipeline that delivers precise, reproducible NAb titers with quantified uncertainty for every result. coreTIAs statistical framework enables robust estimation of neutralization even when dilution series are incomplete, helping to reduce repeat testing and minimizing sample volume requirements. Evaluation and refinement of key assay parameters support consistent performance across AAV serotypes. By providing a protocol and analysis suite as an open resource, coreTIA facilitates more consistent and transparent NAb measurement, potentially aiding assay harmonization and regulatory assessment, addressing a key barrier to progress in gene therapy research and development.
Matching journals
The top 7 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Transcriptomics-informed pharmacology identifies epigenetic and cell cycle regulators as enhancers of AAV production 93%
- Correlating physicochemical and biological properties to define critical quality attributes of a recombinant AAV vaccine candidate 93%
- Combining CRISPR/Cas mediated terminal resolution with a novel genetic workflow to achieve high diversity adenoviral libraries 93%
Similar papers in this journal
- Protocol for capturing the RNA-binding proteome from plants using orthogonal organic phase separation 88%
- Protocol for Developing a Mouse Model of Post Primary Pulmonary Tuberculosis after Hematogenous Spread in Native Lungs and Lung Implants 88%
- Heritable CRISPR-Cas9 editing of plant genomes using RNA virus vectors 87%
Similar papers in this journal
- SARS-CoV-2 mechanistic correlates of protection: insight from modelling response to vaccines 91%
- Cap-independent co-expression of dsRNA-sensing and NF-κB pathway inhibitors enables tunable self-amplifying RNA expression with reduced immunotoxicity 91%
- A statistical framework for quantifying the nuclear export rate of influenza viral mRNAs. 91%
Similar papers in this journal
- Development of a Novel Medium Throughput Flow-Cytometry Based Micro-Neutralisation Test for SARS-CoV-2 with Applications in Clinical Vaccine Trials and Antibody Screening 92%
- Establishment of a well-characterized SARS-CoV-2 lentiviral pseudovirus neutralization assay using 293T cells with stable expression of ACE2 and TMPRSS2 92%
- A novel assessment method for COVID-19 humoral immunity duration using serial measurements in naturally infected and vaccinated subjects 91%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.