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Genome evolution in parthenogenetic nematodes shaped by chromosome rearrangements and rare sex

Chung, G.; Stevens, L.; Dai, X.; Hickey, S.; Kiontke, K.; Rughani, P.; Kieninger, M. R.; Collins, J. C.; Juul, S.; Piano, F.; Fitch, D. H. A.; Blaxter, M.; Gunsalus, K. C.

2025-04-27 genomics
10.1101/2025.04.24.650375 bioRxiv
Show abstract

In contrast to their dioecious relatives, members of the parthenogenetic Diploscapter nematode genus harbour their entire genome within a single pair of highly heterozygous chromosomes. To examine how this unusual karyotype relates to the evolution of parthenogenesis, we generated chromosome-level assemblies for two species in this clade: Diploscapter pachys and Diploscapter coronatus. Sequence comparisons revealed that the two genomes are colinear across their entirety, and that multiple ancestral chromosome fusions and extensive genomic rearrangements preceded the divergence of these two species. The presence of shortened telomeres and extended subtelomeric repeats suggests that the fusions arose from defects in telomere function in the lineage. Our analysis also identified an introgression event after divergence of the two species, suggesting that their parthenogenetic lifestyle may have been punctuated by rare sexual reproduction. These findings shed new light on how telomere loss, chromatin architecture, and reproductive strategies interconnect in shaping chromosome evolution.

Published in Genome Research (predicted rank #4) · training set

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